serum monkey hmw adiponectin  (R&D Systems)

Journal: Acta physiologica (Oxford, England)

Article Title: Restoring adiponectin via rosiglitazone ameliorates tissue wasting in mice with lung cancer.

doi: 10.1111/apha.14167

Figure Lengend Snippet: FIGURE 4 Restoring circulating adiponectin results in increased AMPK activity and improved maintenance of Akt signaling in skeletal muscle of cachectic mice. (A–C) Western blot analysis of CAMKII, AMPK, and p38 signaling in gastrocnemius muscle of cachectic mice (n = 12 per group). The bar graphs represent the ratio of phosphorylated to total protein levels. (D, E) Western blot analysis of Akt activity and downstream signaling in gastrocnemius muscle of cachectic mice (n = 12 per group). The bar graphs represent the ratio of phosphorylated to total protein levels. (F) Whole membrane strips corresponding to the quantified western blot data from “A–E". Data information: Error bars stand for SD. The p-value was calculated by an unpaired t-test. * denotes a p-value of <0.05, ‡ denotes a p-value of <0.001.

Article Snippet: Serum adiponectin (ALPCO Diagnostics, cat no. 47- ADPMS- E01), insulin (Crystal Chem cat. no 90080), and triglycerides (LiquiColor, cat no. 2100- 225) were determined using commercially available kits, per the manufacturer's instructions.

Techniques: Activity Assay, Western Blot, Membrane

Journal: Acta physiologica (Oxford, England)

Article Title: Restoring adiponectin via rosiglitazone ameliorates tissue wasting in mice with lung cancer.

doi: 10.1111/apha.14167

Figure Lengend Snippet: FIGURE 5 Adiponectin activates AMPK and acutely improves muscle protein synthesis in the presence of IL-6, while chronic effects appear insulin-dependent. (A–D) Western blot analysis of AMPK activity, anabolic signaling, and protein synthesis in C2C12 cells after an acute dose–response experiment (n = 4 per group). Cells were treated with increasing doses of adiponectin (10, 25, 50 μM) together with IL-6 (10 ng/mL) for 3 h. The control condition was untreated, the IL-6 condition received only IL-6 (10 ng/mL), and the Drug Ctrl group received only adiponectin (25 μM). “A” shows representative pictures of the graphs from “B–D". Phosphorylated levels of AMPK (B), S6K1 (C), and puromycin (D) were normalized to total protein content per lane. (E–H) Western blot analysis of AMPK activity, anabolic signaling, and protein synthesis in C2C12 cells after chronic exposure to adiponectin or rosiglitazone (n = 3 per group). Cells were treated with adiponectin (25 μM) or rosiglitazone (10 μM) together with IL-6 (10 ng/mL) for 24 h. Thirty minutes before collection, half of the cells were treated with 100 nM insulin. The Ctrl group was untreated, the IL-6 group only received IL-6 (10 ng/mL). “E” shows representative pictures of the graphs from “F–H". Phosphorylated levels of AMPK (F), S6K1 (G), and puromycin (H) were normalized to total protein content per lane. Data information: Error bars stand for SD. The p-value was calculated by a one-way ANOVA and Dunnett's multiple comparison test (acute experiment, Figure 5A–D), and a two-way ANOVA and Sidak's multiple comparison test (chronic experiment, Figure 5E–H). * denotes a p-value of <0.05.

Article Snippet: Serum adiponectin (ALPCO Diagnostics, cat no. 47- ADPMS- E01), insulin (Crystal Chem cat. no 90080), and triglycerides (LiquiColor, cat no. 2100- 225) were determined using commercially available kits, per the manufacturer's instructions.

Techniques: Western Blot, Activity Assay, Control, Comparison

Journal: International Journal of Exercise Science

Article Title: A Short-Term Paleolithic Dietary Intervention Does Not Alter Adipokines Linked to Adiposity

doi:

Figure Lengend Snippet: Adiponectin (A), Omentin (B), and Nesfatin (C) concentration (means and SE’s) before and following eight weeks of adherence to the Paleolithic diet (n=7)

Article Snippet: Serum HMW adiponectin (30 kDa adipocyte complement-related protein; intra-assay CV 8.9%) was measured using a commercially available ELISA kit (RayBiotech, Norcross, GA, USA).

Techniques: Concentration Assay